①墨角藻中的巖藻多糖具有很高的抗腫瘤活性；②巖藻多糖能夠有效地阻止EGF引起的癌細(xì)胞轉(zhuǎn)移；③體外實(shí)驗(yàn)表明，巖藻多糖通過抑制絲裂原活化從而抑制結(jié)腸癌細(xì)胞生長(zhǎng)；④巖藻多糖直接作用于TOPK激酶，抑制TOPK的活性，從而阻止結(jié)腸癌細(xì)胞的轉(zhuǎn)移和增殖。

延伸閱讀

Oncotarget, 2016, 7 (14):18763-18773.

PDZ-binding kinase/T-LAK cell-originated protein kinase is a target of the fucoidan from brown alga Fucus evanescens in the prevention of EGF-induced neoplastic cell transformation and colon cancer growth

Abstract:

The fucoidan with high anticancer activity was isolated from brown alga Fucus evanescens. The compound effectively prevented EGF-induced neoplastic cell transformation through inhibition of TOPK/ERK1/2/MSK 1 signaling axis. In vitro studies showed that the fucoidan attenuated mitogen-activated protein kinases downstream signaling in a colon cancer cells with different expression level of TOPK, resulting in growth inhibition. The fucoidan exerts its effects by directly interacting with TOPK kinase in vitro and ex vivo and inhibits its kinase activity. In xenograft animal model, oral administration of the fucoidan suppressed HCT 116 colon tumor growth. The phosphorylation of TOPK downstream signaling molecules in tumor tissues was also inhibited by the fucoidan. Taken together, our fndings support the cancer preventive effcacy of the fucoidan through its targeting of TOPK for the prevention of neoplastic cell transformation and progression of colon carcinomas in vitro and ex vivo.

First Authors:

Olesia S. Vishchuk, Huimin Sun

Correspondence:

Wei Yan, Qiuhong Duan, Feng Zhu

All Authors:

Olesia S. Vishchuk, Huimin Sun, Zhe Wang, Svetlana P. Ermakova, JuanJuan Xiao, Tao Lu, PeiPei Xue, Tatyana N. Zvyagintseva, Hua Xiong, Chen Shao, Wei Yan, Qiuhong Duan, Feng Zhu

2016-02-25 Article